Objective To study the method of improving the success rate of amniotic fluid cell culture. Method Amniocentesis was performed in B-ultrasound, and 2000 cases of amniotic fluid were extracted. After centrifugation, the pellets were inoculated into culture flask and cultured in 37℃ 5% CO2 cell incubator. Results The best way to improve the success rate of amniotic fluid cell culture was to improve the cell culture environment by optimizing the culture method. The success rate was 99.9%. Conclusions Rapid and timely treatment of amniotic fluid specimens, control the environment and conditions of culture, accurately grasp the cell harvest time can improve the success rate of amniotic fluid culture.
