分类号：R394.2
出版年·卷·期（页码）：2013·5·第2期（12-14）
DOI:
20130204
-----摘要：-------------------------------------------------------------------------------------------
目的 建立稳定的羊水及绒毛细胞原位培养方法。方法 采用细胞原位培养载玻片法，对100例羊水和30例绒毛标本进行原位培养收获，制备好的玻片于GSL-120染色体全自动扫描系统进行克隆识别扫描。结果 结合染色体全自动扫描平台，建立了条件稳定、操作简单、分析过程简化的原位培养载玻片法。100例羊水标本均培养成功，其中异常4例，核型分别为2例46,XX,inv(9)，1例47,XX,+21，1例45,XY,rob(13;14)；30例绒毛标本中，10例为介入性产前诊断标本，均培养成功，核型均正常；余20例为流产绒毛，成功17例，其中5例异常，核型分别为2例47,(XX/XY),+16，1例47,XX,+14，1例45,X，1例47,XX,+3。结论 细胞原位培养载玻片法培养周期短，操作简便，染色体形态好，核型多且完整，配合染色体全自动扫描系统，大大缩短阅片时间，能更加及时准确的得出产前诊断结果。
-----英文摘要：---------------------------------------------------------------------------------------
Objective To establish a stable technique for amniotic fluid and chorionic villus cell culture in situ. Method Using the method of cell in slides culture in situ,100 cases of amniotic fluid and 30 cases of chorionic villus were harvested and the prepared slides were placed on the GSL-120 chromosome automatic scanning system for clone identification and scanning. Results Combined with chromosome automatic scanning system, we have established a stable and simple-processed method of cell culture in situ with slides.100 cases of amniotic fluid all had successful culture, and 4 cases of abnormal karyotypes were found, including 2 cases of 46,XX,inv(9),1 cases of 47,XX,+21,and 1 cases of 45,XY,rob(13;14).Of the 30 cases of chorionic villus,10 were invasive prenatal diagnosis specimens, which were all successfully cultured and had normal karyotypes. Other 20 chorionic villus specimens were from abortion cases, among which 17 cases had successful culture, and 5 cases of abnormal karyotypes were found, including 2 cases of 47,(XX/XY),+16，1 cases of 47,XX,+14,1 cases of 45,X,and 1 cases of 47,XX,+3. Conclusions The method of cell in slide culture in situ has shorter culture time, more convenient operation, and more complete karyotypes available for analysis, With the chromosome automatic scanning system, we have greatly shorten the karyotype analysis time and the diagnosis results can be sent out more timely and accurately.
-----参考文献：---------------------------------------------------------------------------------------
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吴小青,李英,谢晓蕊,安刚,王燕,徐两蒲,林娜,何德钦,林元.细胞原位培养载玻片法在产前诊断中的初步应用.中国产前诊断杂志,2013,5(2):12-14.
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